Fig. 5

a. Similarity plots of C3 nuclei based on single nucleus sequencing data. Color coding indicates level of relatedness among the nuclei. A sharper contrast between the groups means that the nuclei are more different, while patches of differing colors within the groups indicate similarities to nuclei of the other group (meaning the other MAT locus). Nuclei are grouped based on which MAT locus they contain. b Examples of genotypes of C3 nuclei not consistent with mating type. Indicated in blue the allele typically found in MAT-1 nuclei (ie. the reference allele called in the assembly), in red the allele typically found in MAT-2 nuclei (alternate allele). The MAT locus identity of the different nuclei is indicated on top. In a true dikaryotic division, all MAT-1 nuclei should have blue alleles, while all MAT-2 nuclei should have red alleles. A complete list of putative recombination sites is given in Additional file 8