Figure 2

Heatmap comparison of CDSs in the Flagellar Glycosylation Island (FGI). Top: See Figure 1 for explanation of heatmap format. Strains are ordered by FGI CDS data; dendrogram and strain names at right are coloured (as for Figure 1) for type of neurotoxin formed. Filled triangles (bottom of heatmap): approximately 10 CDS intervals (data for some CDSs are absent). CDSs of FGI-I (CBO2666 – CBO2692) are highly conserved and those of FGI-II (CBO2696 – CBO2729) are less so. Hybridisation profiles divided strains into 6 divisions, numbered at left. Mass of glycans detected by mass spectrometry analysis of FlaA proteins are symbolized in boxes at left. Strains examined by top down mass spectrometry are marked with a filled triangle. Top down profiles of flagellin from strains marked with a single asterisk and the complete structure of the posttranslational modification for strain FE9909ACS (hatch symbol) have been combined with previously published data [31]. Bottom: FGI sequence comparison of proteolytic C. botulinum strains ATCC 3502 (FGI division 2), top, and Langeland type F (FGI division 3), below, confirms heatmap data. Synteny within FGI-I region (left) contrasts markedly with FGI-II; here ATCC 3502 contains approximately 20 CDSs not found in Langeland and displays less synteny and homology with the CDSs of the Langeland FGI-II. Heatmap data show that Langeland FGI contains many of the genes found in FGI-I of ATCC 3502 (yellow columns) while is still missing a large number of genes found in FGI-II of ATCC 3502 (blue columns).