Parallel DNA pyrosequencing unveils new zebrafish microRNAs

BMC Genomics

Table 1 Distribution of microRNA reads in developmental and tissue samples.

	Development stages	Mature organs	Total
Nr reads (perfect match to the genome)	20514	26390	46904
Nr non-miRNA reads	2610	7305	9915
Nr miRNA reads	17904	19085	36989
Nr known miRNA reads	17682	18984	36666
Nr putative new miRNA reads	222	101	323

Approximately 90% of the pyrosequencing reads matching the ZF genome corresponded to mature miRNA reads, 2% were novel miRNAs. The distribution of pyrosequencing reads was similar in developmental (87%) and adult tissue (78%) samples.

ISSN: 1471-2164