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Table 3 Of 115 different Molecular Functions identified in the Gene Ontology analysis, 16 differed significantly in expression between normalized and native libraries.

From: Next-generation pyrosequencing of gonad transcriptomes in the polyploid lake sturgeon (Acipenser fulvescens): the relative merits of normalization and rarefaction in gene discovery

Molecular Function

Total counts normalized

% of counts

Total counts native

% of counts

P-value

actin binding

7

1.13

24

0.30

*

ATP binding

101

16.29

152

1.88

***

cytochrome c oxidase

78

12.58

155

1.92

***

DNA binding

23

3.71

76

0.94

***

GTP binding

6

0.97

22

0.27

*

isomerase activity

4

0.65

8

0.10

**

kinase activity

4

0.65

5

0.06

**

motor activity

4

0.65

0

0.00

***

oxygen binding

12

1.94

2

0.02

***

protein binding

178

28.71

1348

16.71

**

ribosome biogenesis

0

0.00

811

10.05

***

RNA binding

33

5.32

1482

18.37

***

structural constituent of ribosome

0

0.00

1791

22.20

***

sugar binding

2

0.32

6

0.07

**

ubiquinol-cytochrome-c reductase activity

22

3.55

6

0.07

***

zinc ion binding

136

21.94

725

8.99

***

  1. P-value significance; * = 0.05, ** = 0.01, *** = 0.001.