Figure 5

OCT4 core regulatory network. Core OCT4 transcriptional regulatory network identified by the integrative analysis of the re-analysed ChIP-on-Chip data, the OCT4 RNAi knock-down and the sequence-based octamer and sox-oct motif mapping. Green boxes represent genes associated with differentiation and red boxes indicate genes being specific for hESCs as annotated by several further public sources [16–18, 26]. For white boxed genes no detailed annotation about differentiation or stemness characteristics was found by literature research. The network also incorporates the information on direct target genes from the re-analyzed SOX2 (red lines) and NANOG (blue lines) ChIP-on-chip experiments.