Table 5 List of oligonucleotide primers used in this study
Primer | Sequence | Positions (bp) | Expected length (bp) |
|---|---|---|---|
TvMULE1_F | 5'-AAGCGAGCATGAACTGCATCA | 229–249 | 696 |
TvMULE1_R | 5'-TTCCGATCAAGGTCCGGCAATTA | 902–924 |  |
TvMULE2_F | 5'-GCTGACTGTGCGCTAAACATTGCT | 1055–1078 | 544 |
TvMULE2_R | 5'-GCTCAACAATCTGATTACCTGCCC | 1575–1598 |  |
TvMULE3_F | 5'-GGGTATCAAAGAACAAGAGTCACC | 1,286–1,309 | 630 |
TvMULE3_R | 5'-TCTCTTTCAGCGGCTGTCCATCTT | 1,892–1,915 |  |
TvMULE4_F | 5'-GGACAAACTCGCCCATTGTCACTT | 8–31 | 584 |
TvMULE4_R | 5'-TCTTGACAGGTGGATGCTTCGCTA | 568–591 |  |
TvMULE4_2F | 5'-TTCGCCTTTCTGGGAAGTACTGGT | 485–508 | 520 |
TvMULE4_2R | 3'-GTCACTGGCAAATTCGCGGAATCA | 981–1,004 |  |
β tubulin_F | 5'-ACACTCCTTCTCAACAAGCTCCGT | 692–715 | 673 |
β tubulin_R | 5'-AGGCTGTTGTGTTGCCGATGAATG | 1341–1364 |  |