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Table 3 Comparison of microarray and qRT-PCR measurements for genes encoding focal adhesion and ECM components

From: Gene expression profiles in mouse embryo fibroblasts lacking stathmin, a microtubule regulatory protein, reveal changes in the expression of genes contributing to cell motility

Symbol

Microarray

RT-PCR Array

Ctnnb1

-0.90

-4.59

Itgb2

-1.10

-4.32

Spock1

-0.89

-3.61

Adamts1

-0.47

-3.21

Mmp10

-1.27

-2.80

Itgav

-1.33

-1.85

Entpd1

-1.45

-1.77

Itga3

-2.05

-1.47

Itgb4

-1.69

-1.20

Ctnna1

1.96

1.78

Mmp11

1.95

2.04

Col6a1

1.92

2.21

Adamts2

3.34

2.67

Cdh3

1.62

4.34

Selp

1.65

7.29

Thbs1

1.26

-2.05

Postn

3.61

-1.43

Mmp14

-0.66

1.56

Sgce

-0.92

1.80

Cdh4

-1.36

2.42

Adamts5

-1.33

4.61

  1. The qRT-PCR measurements were made from a commercial 96-well plate format. Data for qRT-PCR is shown as the mean of duplicate assays. The top 15 entries show mRNAs with matching directionality of expression fold change between RT-PCR and microarray. The last 6 entries list MRNAs in which expression fold change measured by RT-PCR is in the opposite direction of the microarray data.
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BMC Genomics

ISSN: 1471-2164

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