Influence of genetic background on the occurrence of chromosomal rearrangements in Saccharomyces cerevisiae

BMC Genomics

Table 2 Yeast strains used in this study.

Strains	Genotype	Origin
BY4711	MATα trp3 Δ63	Brachmann et al., 1998 [31]
BY4714	MATa his3 Δ200	Brachmann et al., 1998
Y16546	BY4742 MATα his3 Δ1 leu2 Δ0 lys2 Δ0 ura3 Δ0 YMR106c::KANMX4	EUROSCARF collection Brachmann et al., 1998
Y11781	BY4742 MATα his3 Δ1 leu2 Δ0 lys2 Δ0 ura3 Δ0 YOR005c::KANMX4	EUROSCARF collection Brachmann et al., 1998
Y13756	BY4742 MATα his3 Δ1 leu2 Δ0 lys2 Δ0 ura3 Δ0 YDL059c::KANMX4	EUROSCARF collection Brachmann et al., 1998
Y10540	BY4742 MATα his3 Δ1 leu2 Δ0 lys2 Δ0 ura3 Δ0 YML032c::KANMX4	EUROSCARF collection Brachmann et al., 1998
aUHT	MATa ura2_15,30,72 his3 Δ200 trp1 Δ63	This study
aΔ *yku*	MATa ura2_15,30,72 his3 Δ200 trp1 Δ63 YMR106c::KANMX4	This study
aΔ *lig4*	MATa ura2_15,30,72 his3 Δ200 lys2 Δ0 YOR005c::KANMX4	This study
aΔ *rad52*	MATa ura2_15,30,72 his3 Δ200 lys2 Δ0 YML032c::KANMX4	This study
aΔ *rad59*	MATa ura2_15,30,72 his3 Δ200 trp1 Δ63 YDL059c::KANMX4	This study

The diploid obtained from a cross between BY4711 and BY4714 was sporulated and one spore was transformed with the ura2_15,30,72 allele thus leading to the aUHT strain. All the BY strains are direct descendants of the FY2 strain which itself is directly descended from S288c [31].
Single deletion mutants were constructed by crossing aUHT with strains from the EUROSCARF deletion collection (Y16546 (Δyku80), Y11781(Δlig4), Y10540 (Δrad52) and Y13756 (Δrad59)). The diploids were then sporulated and one spore with the expected phenotype was selected.

ISSN: 1471-2164