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Table 2 List of validated snip-SNPs by RFLP assays.

From: A toolkit for rapid gene mapping in the nematode Caenorhabditis briggsae

Chr SNP id Location (cM) Ultra_contig RE DNA fragments in AF16 (bp) DNA fragments in HK104 (bp) Validation type
      Expected Observed Expected Observed  
1 cb15251 13.45 cb25.fpc4321 DraI 747 700 332 and 415 310 and 400 *
  cb55627 21.82 cb25.fpc3441 HindIII 691 691 251 and 440 270 and 440 *
  cb55670 21.82 cb25.fpc3441 DraI 312 and 437 749 749 312 and 437 **
  cb650 43.93 cb25.fpc4140 SacI 689 689 257 and 432 257 and 432 *
  (bhP27)         
2 cb41028 11.88-13.1 cb25.fpc0011 DraI 332 and 419 332 and 400 751 800 *
  cb43091 21.28-26.58 cb25.fpc0058 HindIII 748 800 365 and 383 383 and 383 *
  cb64777 27.92-33.95 cb25.fpc4206 DraI 325 and 424 325 and 400 749 749 *
3 cb20723 12.46 cb25.fpc4153 DraI 20, 196, 220 and 311 230, 311 and 390 20, 196 and 531 230 and 800 **
  cb54953 21.77 cb25.fpc2976 HindIII 751 800 368 and 383 380 and 400 *
  cb40003 31.14 cb25.fpc0002 DraI 685 PCR failure 208 and 477 800  
4 cb8971 7.88 cb25.fpc4250 DraI 750 PCR failure 337 and 413 350 and 413  
  cb48850 20.02 cb25.fpc1570 HindIII 750 780 314 and 436 350 and 500 *
  cb56202 37.52-41.16 cb25.fpc3835 DraI 60, 190 and 489 60, 190 and 500 60 and 679 60 and 800 *
5 cb39304 20.98-22.23 cb25.fpc4126 HindIII 742 790 286 and 456 310 and 470 *
  cb39354 20.98-22.23 cb25.fpc4126 DraI 751 PCR failure 269 and 482 PCR failure  
  cb62 17.07-17.74 cb25.fpc4470 DraI 54, 295 and 400 PCR failure 54 and 695 800  
  bdP3 unknown cb25.fpc0156 DraI 907 907 227 and 680 227 and 680 *
x cb20148 9.52 cb25.fpc0045 DraI 660 PCR failure 171 and 489 PCR failure  
  cb40232 20.1-20.71 cb25.fpc0003 HindIII 237 and 473 300 and 473 710 850 *
  cb6050 24.07 cb25.fpc4403 DraI 750 PCR failure 270 and 480 290 and 510  
  1. The snip-SNPs are arranged by chromosome and location. The corresponding ultracontigs are also listed. The expected and observed DNA fragments refer to products based on in silico analysis and actual experiments, respectively. The bdP3 location is unknown because the corresponding contig is unassembled. The validation type column marks snip-SNPs that were consistent with prediction (*) and those that differed significantly (**). RE: Restriction enzyme used to digest PCR amplified products.