Figure 3

Observed vs. Expected frequencies of indexes in pooled samples. Vertical bars represent the numbers of high-quality read pairs that were observed (black), compared with the number expected (white) for each index in two experiments. Expected numbers were calculated from the total numbers of high-quality read pairs for each experiment and the intended pooling proportions (from left to right: 50%, 50% for the Replication experiment; and 4%, 4%, 4%, 16%, 72% for the Pooling experiment). The individuals sampled (A through D) and the index sequences used are shown above the corresponding bars.