Figure 4

Similarity of Melampsora homologous gene groups (HGGs) to the proteomes of other basidiomycetes. Similarity of Melampsora A) non-secreted and B) secreted homologous gene groups (HGGs) to the proteomes of Puccinia graminis f. sp. tritici, Ustilago maydis and Sporobolomyces roseus. For each of the 369 Melampsora HGGs, a BLASTX search was performed against the proteomes of the other three species and SimiTri was used to plot the sequence similarity relationships between 417 consensus sequences derived from these HGGs and related species. Each tile in the graphics represents a unique consensus sequence and its relative position is computed from the raw BLAST scores derived above (with a cutoff of > 40). Hence, each tile's position indicates its degree of sequence similarity to each of the three selected databases. Sequences showing similarity to only one database are not shown. Sequences showing sequence similarity to only two databases appear on the lines joining the two databases. The position of positively selected HGGs is indicated by arrows. Tiles are colored by their highest BLASTX score for each of the databases: red ≥ 300; yellow ≥ 200; green ≥ 150; blue ≥ 100; and purple < 100.