Figure 2
From: Mating alters gene expression patterns in Drosophila melanogaster male heads
Candidate genes are expressed in fat tissue. Antisense (A-D,I,K,M-Q) or sense (E-H,J,L,R-V) RNA probes were designed to cDNA clones for CG4825 (A,E,M,R), CG8449 (B,F,I,J,N,S), bgm (C,G,O,T), Prx2540-2 (D,H,K,L,P,U), and CG13360 (Q,V). In situ hybridization to whole-mount tissue showed candidate gene expression in male CS fat body tissue (arrows) present on head (A-H) and abdominal (M-V) cuticle. Purple reactivity indicates presence of transcripts. Brains (I-L) showed light pink background staining but lacked detectable expression of either CG8449 (I,J) or Prx2540-2 (K,L).