Table 2 Functional annotation of putative proteins secreted in the hen's uterus

Ovocalyxin-21 (IPI00574331)

Eggshell specific protein containing a Brichos domain

Podocalyxin (O00592)

Sialoprotein highly negative charged containing a podocalyxin (CD34 antigen) domain. Involved in renal filtration, associated with cancers

Metalloproteinase inhibitor 2 (TIMP2) (O42146)

Metalloproteinase inhibitor, NTR (netrin) domain. Tissue remodelling, complexes with metalloproteinases and irreversibly inactivates them

Lysosomal alpha-mannosidase (O46432)

Alpha mannosidase middle domain, Glycosyl hydrolases family 38 N- and C-terminal domains, which participate in the maturation of N-glycans

ICOS ligand (O75144)

Proteins with domains analog to immunoglobulins (Ig-like C2-type, Ig-like V-type), immunoglobulin superfamily. BTN/MOG family. Important for protein folding

Slit homolog 3 and 2 proteins (O88280; O94813)

Embryonic development and calcium-binding protein containing CTCK (C-terminal cystine knot-like), Epithelial Growth Factor (EGF)-like, laminin-like LRR (leucine-rich) repeat, Calcium-binding EGF-like domains

L-dopachrome tautomerase (O93505)

Involved in the formation of pigments, binds 2 copper ions (Common central domain of tyrosinase)

Serum amyloid A protein (P02740)

Apolipoprotein of the HDL complex. Major acute phase reactant protein containing a serum amyloid A protein domain

Tissue alpha-L-fucosidase (P04066)

Alpha-L-fucosidase putative active site involved in glycoprotein metabolism

Endoplasmin (P08110)

Molecular chaperone that functions in the processing and transport of secreted proteins. Contain ATP-binding region, calcium ion binding, heat shock protein 90 (HSP90)-like ATPase domains

Apolipoprotein A-I (P08250)

Lipid transport protein. Apolipoprotein A1/A4/E domain

Alpha-2-antiplasmin (P08697

Serine protease inhibitor (serpin)

Lysosomal protective protein (P10619)

Protective protein with a carboxypeptidase activity

Beta-2-microglobulin (P21611)

Essential subunit of major histocompatibility complex class I molecules containing Ig-like domains.

Osteopontin (P23498)

Glycoprotein of bone, eggshell, kidney and various body secretions. Involved in the mineralization of the shell

Neuronal pentraxin-2 (P47972)

Calcium binding protein involved in acute immunological responses. Contains a concanavalin-A lectin domain.

Glioma pathogenesis-related protein 1 (P48060)

Belongs to the CRISP family. Contain allergen V5/Tpx-1 related, SCP-like extracellular (Ca++ chelating serine protease) domains

Glycine receptor subunit beta (P48167)

Neurotransmitter-gated ion channel. Increase chloride conductance

Receptor-type tyrosine-protein phosphatase-like N (P56722)

Implicated in neuroendocrine secretory processes

Amyloid beta A4 protein (P79307)

Protein associated with Alzheimer disease containing an heparin-binding and Kunitz/bovine pancreatic trypsin inhibitor domains

Nucleobindin-2 (P80303)

Calcium binding proteins containing an EF-hand domain

Neuroplastin (P97300)

Cell adhesion molecule of the immunoglobulin superfamily. It contains Ig-like domains (Ig-like C2 and V types)

Golgi apparatus protein 1 (Q02391)

Receptor for Fibroblast Growth Factors (FGF) containing cysteine-rich GLG1 repeat

Follistatin-related protein 1 (Q12841)

Involved in cell proliferation, differentiation and survival. Containing calcium-binding EF-hand, a protease inhibitor, kazal type and von Willerbrand factor domains

FK506-binding protein 9 (Q2KJC8)

FKBP-type peptidyl-prolyl cis-trans isomerase involved in folding of proteins during protein synthesis. Contains calcium-binding EF-hand domain.

UPF0577 protein KIAA1324-like homolog (Q3UZV7)

Protein containing nine Cystein Domains of family 3 GPCR

Ovocalyxin-36 (Q53HW8)

Specific chicken eggshell matrix protein with antimicrobial activity Potentially involved in the mineralization of the eggshell. Contains LBP/BPI/CETP family, N-terminal domain

Glutamate [NMDA] receptor subunit zeta-1 (Q5R1P0)

Receptor family ligand binding region, Calmodulin-binding domain C0 of NMDA receptor NR1 subunit, Ligand-gated ion channel

Calnexin (Q5R440)

Molecular chaperone and calcium binding protein, which interact to newly synthesized glycoproteins to play a role in protein folding

Butyrophilin subfamily 1 member A1 (Q62556)

Specific membrane-associated receptor. Contains CD80-like C2-set immunoglobulin domain, B302 (SPRY) domain and Ig-like V-type (immunoglobulin-like) domain

UDP-glucuronosyltransferase 1-1 (Q63886)

UDP-glucoronosyl and UDP-glucosyl transferase family. Involved in detoxication and elimination of toxics

Mannose-binding protein C (Q66S61)

Binds mannose and N-acetylglucosamine in a calcium-dependent manner. Is capable of host defense. Contains Collagen triple helix repeat, Lectin C-type domain

Renin receptor (Q6AXS4)

Renin receptor-like protein

Avian Beta Defensin 9 (Q6QLR1)

Beta defensin family having bactericidal activties

Protein shisa-2 homolog (Q6UWI4)

Plays an essential role in the maturation of presomitic mesoderm cells by individual attenuation of both FGF and WNT signalling

Tissue factor pathway inhibitor 2 (Q7YRQ8)

Protease inhibitor (Kunitz/Bovine pancreatic trypsin inhibitor (BPTI) domain) involved in tissue modelling

Ganglioside GM2 activator (Q8HXX6)

Contains MD-2-related lipid-recognition domain involved in innate immunity and lipid metabolism

Dentin matrix protein 4 (Q8IXL6)

Calcium-binding protein, which may play a role in dentin mineralization

BMP-binding endothelial regulator protein (Q8N8U9)

Inhibitor of bone morphogenetic protein (BMP). Contains C8 domain, Trypsin Inhibitor like cysteine rich domain, von Willebrand (VWFC) factor type C domain and type D (VWFD) domains

78 kDa glucose-regulated protein (Q90593)

Heat shock protein (Hsp) 70 family. Probably plays a role in facilitating the assembly of multimeric protein complexes

Chordin (Q91713)

Involved in embryonic development via BMP2/4. Contains CHRD (chordin) and von Willebrand (VWFC) factor type C domains

EMI domain-containing protein 1 (Q91VF5)

Involved in tissue remodelling. Contains collagen triple helix repeat domain, EMI domain

Interleukin-17 receptor A (Q96F46)

Receptor for IL17A containing SEFIR domain

Calsyntenin-3 (Q99JH7)

Involved in cell adhesion, synaptic transmission, may modulate calcium-mediated postsynaptic signals. Contains cadherin domain

UPF0556 protein C19orf10 homolog (Q9CPT4)

Uncharacterised protein family UPF0556

Beta-amyloid protein 751 isoform (Q9DGJ7)

Protein containing Amyloid A4 extracellular, Beta-amyloid peptide, Kunitz/Bovine pancreatic trypsin inhibitor domains

Anthrax toxin receptor 1 (Q9H6X2)

Protein with Anthrax receptor C-terminus region, Anthrax receptor extracellular and von Willebrand factor type A (VWFA) domains

Thioredoxin domain-containing protein 16 (Q9P2K2)

Potentially involved in cell redox homeostasis

Ovocleidin-116 (Q9PUT1)

Chicken eggshell matrix protein constituting the major core of eggshell proteoglycan. May be involved in the mineralization process

Protein sel1 homolog 1 (Q9UBV2)

Sel1 family protein. May play a role in Notch signalling. Contains fibronectin type-II domain and Sel1-like repeats

Spondin-1 (Q9W770)

Cell adhesion protein containing Reeler, spondin-N and thrombospondin type 1 domains

Angiopoietin-related protein 3 (Q9Y5C1)

Fibrinogen beta and gamma chains, C-terminal globular domain protein

Beta-secretase 2 (Q9Y5Z0)

Aspartyl protease with broad endopeptidase specificity