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Table 4 Comparison of expression levels (log2ratio) from qRT-PCR and microarray for selected target genes

From: Development and validation of a flax (Linum usitatissimum L.) gene expression oligo microarray

Target Transcript

Gene ID

Microarray value

(Log2ratio)

qRT-PCR value

(Log2ratio)

Lipoxygenase LOX2

c20715

7.86465

17.6

Chlorophyll a-b binding protein 3C-like

c2491

3.9446

7.47

RuBisCO activase 2

c602

4.5263

12.185

DNA-directed RNA polymerase II

c21991

0.01865

-0.015

Calmodulin TaCaM2-1

c24118

-0.10225

0.295

Ubiquitin carboxyl- terminal hydrolase

c9380

0.05875

0.215

Cellulose synthase

c2533

-4.5911

-7.37

Laccase

c3323

-6.3722

-7.31

Fasciclin-like AGP 4

c2370

-6.7035

-9.72

  1. Gene expression was calculated as log2ratio of L vs SIGC samples, using mean signal intensity across three technical replicates of each sample. Values >1 indicate up-regulation, and values <-1 indicate down-regulation.