Figure 4

Blotting analysis of 39 3FTx probe-positive BAC clones. 3FTx probe-positive BAC clones were digested with Eco R I and analyzed on 0.8% agarose gels prior to blotting on a nylon membrane (upper panel). The 3FTx gene-containing fragments were identified by hybridization with digoxigenin-labeled 3FTx probes (lower panel). The positive bands were used as a control to cut and purify 3FTx gene-containing fragments in a second gel. Lanes: M, 1 kb DNA ladder; 1-39, 3FTx probe-positive BAC clones (Additional file 2: Table S2).