Figure 4

Distribution of several SSRs in the D. melanogaster cytogenetic reference map of the heterochromatic regions (h1 to h61) derived from Pimpinelli et al. [26] (the darker the block, the brighter the DAPI intensity; centromeres [C] and rDNA are also indicated). At the resolution in Figure 1, the distribution of the pentanucleotide repeats is the same as reported by Lohe et al. [10] and Mukani et al. [11]. Thus, the localizations of these pentanucleotide SSRs are indicated below the chromosomes as previously mapped. Additional localizations to those reported are shown by arrows. Lines above chromosomes represent the novel SSRs described in the present work (Figs. 2 and 3). Note that some sequence has been mapped to chromosome regions defined by several heterochromatic bands, but neither the physical size nor intensity of the fluorescence signals is represented here.