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Table 2 qRT-PCR validation of 10 differentially expressed genes according to microarray experiment

From: Identification of genes differentially expressed in a resistant reaction to Mycosphaerella pinodes in pea using microarray technology

Oligo IDa

TIGR IDb

Annotation

Haic

Mdmicroarray

M qRT-PCRe

MT014704

TC80412

Peroxidase

48

1.56

2.99

MT006316

TC82368

Disease resistance response protein 39 precursor

16

1.29

0.91

MT014356

TC85808

12-oxophytodienoate reductase (OPR2)

16

1.27

-1.62

MT014726

TC79559

glutathione S-transferase

16

1.96

2.25

MT007682

TC77455

nine-cis-epoxycarotenoid dioxygenase1

16

1.12

0.21

MT000707

TC86358

6a-hydroxymaackiain methyltransferase

16

-2.13

-0.03

MT000671

TC86307

ferredoxin--NADP+ reductase

48

-1.2

-2.3

MT014197

TC85300

chlorophyll a/b-binding protein

48

-1.34

-1.33

MT007613

TC86304

GA protein

48

-2.28

-2.65

MT014137

BF633423

ribulose 1 5-bisphosphate carboxylase small subunit

48

-0.92

0.32

  1. a Oligo ID, identifier of M. truncatula 70-mer oligonucleotides
  2. b TIGR ID, identifier in the TIGR M. truncatula Gene Index;
  3. c Hai = hours after inoculation with M. pinodes
  4. d M = log2 expression ratio P665/Messire
  5. e Primers used to amplify the genes are shown in Table 4