Directional RNA deep sequencing sheds new light on the transcriptional response of Anabaena sp. strain PCC 7120 to combined-nitrogen deprivation

Table 2 Unstudied regulatory genes with 5-fold or greater increase in expression by 6, 12, or 21 hours after nitrogen step down¹

		RPKM fold change
Gene name	Gene description	6 h	12 h	21 h
typA (all4140)²	GTP-binding protein TypA/BipA	-1.2	6.8	5.9
all0723²	probable GTP-binding protein	1.1	6.1	6.2
all2564³	pyruvate kinase	2.3	50.5	25.2
all4008³	pyruvate kinase	1.0	4.3	5.3
all0192²	serine/threonine kinase	6.5	45.4	52.8
alr1336³	serine/threonine kinase	1.9	3.2	6.3
all4838³	serine/threonine kinase	1.3	6.6	3.8
alr1044²	transcriptional regulator	1.5	6.1	2.1
all2237²	transcriptional regulator	1.1	5.5	4.6
alr2479³	transcriptional regulator	-1.2	7.9	2.8
alr2769²	transcriptional regulator	-2.5	3.0	8.3
alr3646³	transcriptional regulator	1.1	9.0	2.1
all3728²	transcriptional regulator	2.4	7.2	6.7
alr4564²	transcriptional regulator	1.6	3.9	5.3
alr0546²	two-component sensor histidine kinase	1.0	5.3	2.6
all3359²	two-component sensor histidine kinase	-1.3	5.7	8.4
alr4878²	two-component hybrid sensor and regulator	1.4	6.2	3.8
alr5188³	two-component response regulator	1.1	5.5	2.8

¹Only unstudied genes with at least one-fold read coverage and annotated with the GO term "regulatory function" are presented.
²Genes that were not identified as being significantly upregulated in previous microarray analyses [13, 49].
³Genes that were previously identified as being regulated in microarray data but that show a greater degree of upregulation in our RNA-seq data [13, 49].

ISSN: 1471-2164