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Figure 4 | BMC Genomics

Figure 4

From: Development of a Pacific oyster (Crassostrea gigas) 31,918-feature microarray: identification of reference genes and tissue-enriched expression patterns

Figure 4

Expression stability of reference genes. Results of quantitative RT-PCR analysis are depicted as (A) Ct values measured or (B) Log (mean Ct/Ct sample) for each gene analyzed. Box plots graphically depict the minimum value, lower quartile, upper quartile and maximum value. (C) Coefficient of variation (CV; ratio of the standard deviation and the mean). (D) Average expression stability as calculated using geNorm. For details see Material and Methods. Genes are ordered according to increasing expression stability, identifying hkg4 and g3apdh as the best housekeeping genes for reference.

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BMC Genomics

ISSN: 1471-2164

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