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Table 1 Primary and secondary response 20E-regulated genes identified in Kc167 cells

From: Genome-wide examination of the transcriptional response to ecdysteroids 20-hydroxyecdysone and ponasterone A in Drosophila melanogaster

Group

Gene Symbol

20E Fold Difference

q value (%)

Primary fold difference

q value (%)

Select Functional Annotation Terms Enriched in Group

 

br

3.58

0.00

4.75

0.00

 
 

CG5346

3.10

0.00

2.91

0.00

induction of

 

Rrp46

2.45

0.00

2.68

0.00

programmed cell

 

Pect

2.89

0.00

2.49

0.00

death by hormones;

 

CG14523

1.83

0.40

2.37

0.00

 
 

Cyp9c1

1.87

1.13

2.35

0.00

catalytic activity;

 

granny-smith

1.79

0.00

2.21

0.00

 
 

CG4825

2.10

0.00

2.14

0.00

cytochrome P450,

 

CG17760

2.51

0.00

2.13

0.00

E-class, group I;

E

Eip55E

1.92

0.00

2.03

0.00

 
 

CG15482

2.75

0.00

1.83

0.00

hydrolase activity;

 

Eip75B

1.82

0.00

1.83

0.00

 
 

Nc

1.40

0.00

1.80

0.00

 
 

eater

1.97

0.00

1.76

0.00

 
 

Idgf2

-1.06

 

1.74

0.00

 
 

CG11586

1.00

 

1.74

1.89

 
 

PRL-1

2.10

0.00

1.70

0.00

 
 

l(2)09851

-1.08

 

1.70

0.00

 
 

vri

1.52

0.84

1.68

0.00

 
 

ImpL2

1.42

 

1.68

0.00

 
 

Eip71CD

4.06

0.00

   
 

CG11893

2.28

0.62

  

mitochondrial

 

CG15711

2.20

0.00

  

respiratory chain;

 

CG30104

2.03

0.00

   

F

CG5059

2.00

0.34

  

glutathione

 

CG17819

2.00

0.72

  

S-transferase;

 

CG5104

1.99

0.00

   
 

CG31633

1.89

2.76

  

cellular metabolic

 

CG5694

1.87

4.45

  

process;

 

Obp8a

1.83

3.56

   

G

Aldh

-1.32

3.56

-2.05

2.10

 
 

CG8801

-1.84

0.62

   
 

ncd

-1.92

0.38

  

nucleotie-binding;

 

Hsp70Aa

-2.01

0.49

   
 

Hsp68

-2.13

1.13

  

kinase activity;

H

Scp2

-2.13

4.45

   
 

gammaTub23C

-2.17

1.13

  

glycolysis;

 

Hsp60

-2.43

0.00

   
 

Acon

-2.51

1.76

  

cytoskeleton

 

Vago

-2.57

0.00

  

organization and

 

Hsp70Ab

-2.91

1.13

  

biogenesis

  1. A two- class SAM analysis of triplicate data identified primary-response genes that responded to 20E in the presence of cycloheximide. The top 20 up-regulated primary-response genes and single down-regulated primary-response gene are listed (group E and G respectively). Secondary-response genes regulated by 20E only in the absence of cycloheximide were also identified. The top 10 up- and down-regulated secondary-response genes are listed (groups F and H respectively). The fold change of each gene following a 4 hour treatment with 0.5 μM 20E (20E fold difference) and, for primary-response genes, the fold change following a 4 hour treatment with 0.5 μM 20E and 100 μM cycloheximide (primary fold difference) is given. q-values determined by SAM represent the lowest false discovery rate at which that gene is considered significant. For a gene to be included as differentially expressed, it must have a q-value < 5% in that experimental condition. q-values > 5% are not shown. DAVID (http://david.abcc.ncifcrf.gov/) was used to identify enriched annotation categories among groups of genes that respond similarly to 20E. Some of the most enriched functional terms associated with each group (i.e. terms with modified Fisher Exact p-values ≤ 0.05; where the p-value gives the probability of that term being randomly associated with the genes in the group) are given in the right most column. A complete list of all genes in these groups and their functional annotation is in Additional file 2.
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BMC Genomics

ISSN: 1471-2164

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