Table 4 Assessment of assembly reliability on randomly selected contigs by using PCR with primers designed from BAC end sequences
From: Generation of the first BAC-based physical map of the common carp genome
Contig ID | Number of primer pairs | Number of Clones | Number of Positive Clones | Contig assembly completely validated |
|---|---|---|---|---|
2042 | 15 | 65 | 65 | Yes |
113 | 12 | 85 | 85 | Yes |
2092 | 8 | 34 | 34 | Yes |
348 | 2 | 11 | 11 | Yes |
4368 | 4 | 11 | 11 | Yes |
1494 | 4 | 25 | 25 | Yes |
2220 | 4 | 14 | 14 | Yes |
4074 | 2 | 10 | 10 | Yes |
1258 | 2 | 13 | 13 | Yes |
6064 | 2 | 13 | 13 | Yes |
3806 | 4 | 17 | 17 | Yes |
4498 | 4 | 13 | 13 | Yes |
677 | 6 | 37 | 37 | Yes |
2995 | 4 | 24 | 24 | Yes |
3682 | 4 | 22 | 22 | Yes |
5929 | 2 | 7 | 7 | Yes |
3097 | 2 | 12 | 12 | Yes |
984 | 2 | 12 | 12 | Yes |