Figure 5

Arrayed screening using the lentiviral library. (A) A375 cells were seeded in a 96-well plate and infected with virus containing an empty vector, let-7a, or miR-372. Five days after infection, cells expressing let-7a were significantly less numerous than cells expressing empty vector, while cells expressing miR-372 were significantly more numerous (left panel, * indicates p < 10^-4 in a two-tailed t-test). Values are average number of cells per well ± standard deviation, n = 6. Each measurement of both let-7a and miR-372 samples is separated from the empty vector samples by more than three standard deviations (Z-score, right panel). (B) Results of an arrayed screen on cell viability of A375 cells. Cells in individual wells were infected with single miRNA constructs and subjected to an MTS assay 5 days after infection. The screen was performed in duplicate. For each measurement a B-score was calculated. Correlation between duplicate B-scores is shown. The green dots represent let-7a-1, -2, and -3, while the red dot represents miR-372.