Figure 3

Characterization of the bald cypress BAC library. (A) Size distribution of BAC inserts in the TDD_Ba library as determined by pulsed-field gel electrophoresis (PFGE) of Not I-digested BAC clones. (B) Image of a PFGE gel showing Not I digested BAC clones. The BAC vector is visible as a 7.5 kb band near the bottom of each sample lane. The New England BioLabs Lambda Ladder is shown in the far right lane. (C) Southern blot of the gel shown in B hybridized with bald cypress genomic DNA. Note that some clones exhibit strong hybridization (blue arrows) while others exhibit relatively weak hybridization (red arrows). Clones with strong hybridization signals ostensibly contain more repetitive DNA than those with weak signals.