Erroneous attribution of relevant transcription factor binding sites despite successful prediction of cis-regulatory modules

Table 1 CRM predictions tested in this study

CRM name	coordinates (r5/dm3)	reporter activity	putative target gene	primary expression pattern
CG32105_cooc164	chr3L:12306977-12307990	+	CG32105	ventral nerve cord, amnioserosa
CG34347_cooc404	chr3R:27173861-27175568	+	CG34347	ventral nerve cord (midline*), amnioserosa
cooc110	chr2L:17140480-17141559	+	beat-IIIa	pupal wing, eye, leg and other tissues
cooc437	chr3R:19617439-19618229	-	n/a	none
jhamt_cooc102	chr2L:16364204-16365055	+	jhamt	somatic and visceral muscle from stage 12 onward
notum_cooc310	chr3L:16013542-16014711	+	notum	segmentally repeated stripes, mostly ectodermal with limited mesoderm expression in dorsal regions and in the anal ring

*a shorter version of cooc404 also had ventral nerve cord activity but ectopic relative to CG34347 expression (data not shown)

ISSN: 1471-2164