Figure 4

qRT-PCR validation of differential expression. The figure shows transcript levels of 22 genes, of which 12 probably associated with fruit development and ripening (A) and 10 with oxidative phosphorylation (B), the OPP pathway (C) or carotenoid biosynthesis (D) in MT (solid line) and WT (broken line). The y-axis records the relative gene expression levels analyzed by qRT-PCR. Bars represent the standard error (n = 3). (E) A comparison between the gene expression ratios obtained from RNA-seq data and qRT-PCR. The RNA-seq log₂ of expression ratio (y-axis) have been plotted against developmental stages (x-axis).