| Sequences generated | % |
---|
+S library
| Â | Â |
Total reads | 11284767 | Â |
High quality | 10762852 | 100% |
Adaptor3 null | 3304 | 0.03% |
Insert null | 2815 | 0.03% |
Adaptor5 contaminants | 63608 | 0.59% |
Smaller than 18 nt | 663116 | 6.16% |
PolyA | 17 | 0.00% |
Clean reads | 10029992 | 93.19% |
-S library
| Â | Â |
Total reads | 11080539 | Â |
High quality | 10572756 | 100% |
Adaptor3_null | 3499 | 0.03% |
Insert null | 3575 | 0.03% |
Adaptor5 contaminants | 58280 | 0.55% |
Smaller than 18 nt | 588466 | 5.57% |
PolyA | 6 | 0.00% |
Clean reads | 9918931 | 93.82% |
- Total reads: total sequenced reads; High quality: number of high quality reads with no N, no more than 4 bases whose quality score is lower than 10 and no more than 6 bases whose quality is lower than 13; Adaptor3 null: number of reads with no 3' adaptor; Insert null: number of reads with no insertion; Adaptor5 contaminants: number of 5' contaminants; Smaller than 18 nt: number of reads less than 18 nt. Generally, small RNA tags are between 18 ~ 30 nt long. So too short tags should be removed from data for future analysis; polyA: number of reads wih polyA; Clean reads: number of clean reads after adaptors and contaminants are removed which are used in the following analysis.