Fast skeletal muscle transcriptome of the Gilthead sea bream (Sparus aurata) determined by next generation sequencing

Table 1 Number of reads obtained per experimental condition and their respective Newbler assembly results

Assembler	Parameters	Adult	Fasted	Juvenile 21°C	Juvenile 30°C	Juvenile 11°C	Total assembly
Newbler v2.5	Reads	351895	486993	439734	459853	433264	2711149
	Reads assembled	166460	239792	189314	258331	200313	1157833
	Singletons	19809	24231	19140	21125	24896	96351
	Isotigs	6502	9152	8407	9922	9524	50515
	Isotigs over 100 bp	6254	8595	8242	9554	9267	43461
	Isotig mean lenght (bp)	875	589	936	551	550	454
	N50 (bp)	1092	732	1269	672	642	679
	Isotigs Annotated	2634	2791	3031	3211	3029	10465

Singletons: reads not contained in the final assembly.
Isotig: contigs consistently connected by a set of reads.
N50: The value was computed by sorting all contigs from largest to smallest and by determining the minimum set of contigs whose sizes total 50% of the entire transcriptome.

ISSN: 1471-2164