Figure 4

Integration of in silico data with the T. cruzi molecular karyotype: mapping of chromosome ends to the chromosomal bands separated by PFGE. A. Schematic representation showing the location of chromosome ends Tel 30 and Tel 23 in the homologous chromosomes TcChr39-P and TcChr39-S, respectively. The left panel shows the hybridization of marker ankyrin with the chromosomal bands of clone CL Brener separated by PFGE. The probe hybridized only to chromosomal band XVI, indicating that both homologous chromosomes TcChr39-P and TcChr39-P are located in the same chromosomal band. B. Chromosome ends Tel 34 and Tel 21 are located in chromosome TcChr25-S and Tel 5 in chromosome TcChr25-P. The hybridization of subtelomeric marker glucanolactonase-6PP, found in Tel 21, the prohibitin found in Tel 5 and interstitial markers XM_802850 and XM_800447 with the chromosomal bands separated by PFGE is shown at the bottom. The markers hybridized with chromosomal bands V and IX, indicating that the homologs of chromosome TcCh25 are of different sizes. The maps are to scale and the genomic coordinates are indicated at the beginning and end of each map. Each chromosome end assembly is oriented 5’ to 3’ according to the TriTrypDB annotation. Blue arrows indicate the transcription sense.