Skip to main content

Table 1 Sequence Statistics and RNA qualities

From: Increased sensitivity of next generation sequencing-based expression profiling after globin reduction in human blood RNA

Samples

Non- globin reduced

Globin-reduced

 

1

2

3

4

5

6

1

2

3

4

5

6

RIN

8.1

7.7

7.1

6.9

7.3

8.8

7.6

7.2

6.7

6.9

7

8.2

Tag numbers

29.2.106

13.2.106

32.0.106

11.6.106

13.6.106

10.1.106

16.5.106

28.7.106

15.9.106

15.3.106

15.9.106

14.9.106

Non- aligned

1.27%

5.63%

1.43%

13.80%

5.64%

1.92%

3.64%

2.95%

3.37%

3.29%

3.45%

3.38%

Aligned

89.9%

84.4%

89.9%

80.1%

89.1%

91%

78.3%

78.9%

78.4%

78.9%

77.6%

77.5%

Globin tags

60.1%

52.1%

57.2%

69.6%

76%

71%

0.3%

0.4%

0.4%

0.2%

0.4%

0.1%

  1. Quality of RNA depicted by RNA integrity numbers (RIN) for the 6 non-globin reduced and globin reduced samples. The number of tags, percentages of aligned and non-aligned sequences, and relative number of globin tags are shown as percentage of the total number of aligned tags.
Back to article page

BMC Genomics

ISSN: 1471-2164

Contact us