Figure 5

Analysis of histone modifications at the promoter regions of M. domestica orthologues of eutherian imprinted genes. ( A ) Chromatin immunoprecipitation with H3K4me2 antibody followed by PCR amplification is shown for the IGF2R promoter region; L denotes the 100 bp ladder, I the input, R the sample immunoprecipitated with rabbit serum albumin (i.e. non-specific pull-down), and K4 the sample immunoprecipitated with H3K4me2 antibody. Analysis of enrichment was performed by real-time PCR following chromatin immunoprecipitation with H3K4me2 and H3K9me3 antibodies at the promoter regions of ( B ) IGF2R, ( C ) HTR2A, and ( D ) L3MBTL. The fold change is plotted on the Y-axis, and the distance from the putative transcription start site (0) on the X-axis. The black bars represent H3K4me2, and the grey bars represent H3K9me3.