A moderate increase in ambient temperature modulates the Atlantic cod (Gadus morhua) spleen transcriptome response to intraperitoneal viral mimic injection

Hori, Tiago S; Gamperl, A Kurt; Booman, Marije; Nash, Gordon W; Rise, Matthew L

doi:10.1186/1471-2164-13-431

Table 3 QPCR Primers

From: A moderate increase in ambient temperature modulates the Atlantic cod (Gadus morhua) spleen transcriptome response to intraperitoneal viral mimic injection

Gene		Primer sequence 5’ to 3’	Amplification Efficiency (%)	R²	Amplicon Size (bp)
Deltex3^b	Forward	TCCACCACAAGACCAGCATCA	98.5	0.99	110
Deltex3^b	Reverse	ACTTCACTCGATGCCTTTCGC	98.5	0.99	110
DHX58^c	Forward	ACAGAAGCCATCGCAGAAAT	92.9	0.99	105
DHX58^c	Reverse	TTTTGCAGCACGAATCAAAC	92.9	0.99	105
IκBα^c	Forward	GCCAGCAACTGATAAAGCATC	92.1	0.99	132
IκBα^c	Reverse	GGTCACAGAGGGAGACAGAAAA	92.1	0.99	132
IL-8^c	Forward	GTGTTTCCAGCAGATCACTCG	94.9	0.99	118
IL-8^c	Reverse	TGTTCCACTTGGTGAGGAGTC	94.9	0.99	118
IRAK4^c	Forward	CGTGGATTACAAGATGGATAAGC	95.8	0.99	102
IRAK4^c	Reverse	TCGTCGGGGTCTAAAAAGTC	95.8	0.99	102
IRF1^a	Forward	AGAAGGACGCCAGTCTGTTCAA	88.5	0.99	100
IRF1^a	Reverse	GCGGAAGTTGGCTTTCCATT	88.5	0.99	100
IRF7^a	Forward	GGTCGTCGGAGTTCTTGGAGTT	95.1	0.99	102
IRF7^a	Reverse	CCAAACGACAAGGCCAAATG	95.1	0.99	102
IRF10^a	Forward	CGAGGCGGTAGACCTTGTAG	104.6	0.94	161
IRF10^a	Reverse	GGGCAGGTACAAAGGGAAAT	104.6	0.94	161
ISG15^c	Forward	AGGACCAACAAAGGCTGATG	94.7	0.99	110
ISG15^c	Reverse	CAGCCGTCCGTTAAGGTAGA	94.7	0.99	110
PKR^c	Forward	ATTGCATCAGGTTCCCAATC	94.4	0.99	174
PKR^c	Reverse	GCCGTTACCAGACCAAAATC	94.4	0.99	174
RSAD2^c	Forward	TGTTTCCACACAGCGAAGAC	95.8	0.99	108
RSAD2^c	Reverse	TCCGCCAGAGAAGTTGATCT	95.8	0.99	108
SACS^b	Forward	CTCCCACTGCCAATGTCATTC	88.5	0.99	102
SACS^b	Reverse	TCAAGAAAACGTCCCAAGGC	88.5	0.99	102
SCYA123^c	Forward	GCTCTGGGTCGTGTACCTCT	94.1	1.00	189
SCYA123^c	Reverse	TCTCTCTGGACGAACAAGCA	94.1	1.00	189
STAT1^b	Forward	GCCAATGCCATGTGTTTATG	97.5	0.99	100
STAT1^b	Reverse	ACCTGGAGCAGTTCGTCAGT	97.5	0.99	100
TLR3^c	Forward	CCTGAAACGCAACTCTATCTCC	90.6	0.99	121
TLR3^c	Reverse	GCCATCAAACATACCCTTCTTT	90.6	0.99	121
TLR9^c	Forward	TTGCTCGCCAAAACACTATG	99.5	0.99	150
TLR9^c	Reverse	GGAATCCAGTCCCTCTCCTC	99.5	0.99	150
ZNFX1^b	Forward	ATGCCACTATCGGTGGACAGA	87.3	0.99	108
ZNFX1^b	Reverse	TCAACAGATTATTGCCCTCGG	87.3	0.99	108
ATPS^c	Forward	ACATGGATAAATGGCTTTTTGC	99.6	0.98	155
	Reverse	TTGAAGAAGTAGTGTGGCTGGA

^aThese primers were previously designed and used in Rise et al.[22] but were still quality checked for the present experiment using the reference cDNA as template.
^bThese primers were previously designed and used in Rise et al.[25] but were still quality checked for the present experiment using the reference cDNA as template.
^cThese primers were designed based on ESTs or contigs representing probes that were identified as differentially expressed in the microarray experiment.

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ISSN: 1471-2164

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