Table 2 Plasmids and bacteria used in this work
Plasmids | Relevant genotype/information | Source/reference |
|---|---|---|
pK18mobsacB | sacB, lacZ α, mcs (KmR) | [49] |
pET2 | E. coli–C. glutamicum promoter-probe vector (KmR, promoterless cat gene) | [58] |
pET2sigH | sigH promoter region (550 bp) in pET2 | this work |
pET2rshA | rshA promoter region (301) in pET2 | this work |
pET2sigH4 | P4sigH promoter region (348 bp) in pET2 | this work |
Bacteria | ||
E. coli | ||
E. coli JM109 | end A1, rec A1, gyr A96, thi, hsd R17 (rk–, mk+), rel A1, sup E44, Δ(lac-proAB), F´ tra D36, proAB, laqIqlacZ ΔM15 | [59] |
C. glutamicum | ||
RES167 | restriction-deficient C. glutamicum strain ( ΔcglIM-cglIR-cglIIR) | [47] |
DN2 | RES167 deletion of sigH | [24] |
AS1 | RES167 deletion of sigH-rshA | this work |
ΔrshA | RES167 deletion of rshA | this work |