Figure 4

Development of the core genome of the Pseudonocardiaceae. Using EDGAR, the development of the core genome of the Pseudonocardiaceae was extrapolated by calculating the mean core genome numbers for all possible permutations of genomes (red crosses/line). By non-linear least squares curve fitting, an exponential decay function (dark blue curve and equation) was fitted to the mean core data. A 95% confidence interval was calculated for the fitted model, and the boundaries are displayed (light blue and purple curves). Using the genomes of A. mirum, A. mediterranei, P. dioxanivorans, S. erythraea, S. espanaensis, S. viridis, and T. bispora, a final core genome of approximately 810 genes is predicted, with the current core of the seven analyzed species consisting of 864 genes.