Table 1 HFA and SRSF genome coverage and hit selection
From: Advances in genome-wide RNAi cellular screens: a case study using the Drosophila JAK/STAT pathway
| Â | HFA screen | SRSF screen | ||
|---|---|---|---|---|
| Â | FlyBase r2.0 | FlyBase r5.24 | ||
| Â | Nr Genes | % of genome | Nr Genes | % of genome |
Initial Library coverage | 13,226 | 88.8% | 14,587 | 97.9% |
Genes excluded due to screening errors (Figure 1B, step 2) | 820 | 6.2% | 0 | 0.0% |
Remaining genome coverage | 12,406 | 83.3% | 14,587 | 97.9% |
Genes with significant Average FL/RL (Figure 1B, step 4) | 1,161 | 7.8% | 300 | 2.0% |
Final hits after applying all hit selection rules (Figure 1B, step 6) | 134 | 0.9% | 42 | 0.3% |