Table 6 Functional Annotation Clustering of statistically-significant under-expressed genes in European eel silver males from the river Tiber site (N = 8) in comparison with silver males from the lake Bolsena site (N = 7)

Annotation Cluster 1

Actin alpha 1, skeletal muscle

(Enrichment Score: 6.78)

Actin-related protein 2/3 complex, subunit 3

Chromodomain helicase DNA binding protein 2

Collagen, type I, alpha 1

Collagen, type I, alpha 2

DEAD (Asp-Glu-Ala-Asp) box polypeptide 51

Dynein light chain 2

Eukaryotic translation initiation factor 2 subunit 1 alpha

Eukaryotic translation initiation factor 3 subunit 2 beta

Eukaryotic translation initiation factor 3 subunit J

Heterogeneous nuclear ribonucleoprotein D-like

Isoleucyl-tRNA synthetase

Keratin 8

Mitochondrial ribosomal protein 63

Mitochondrial ribosomal protein L13

Mitochondrial ribosomal protein L15

Mitochondrial ribosomal protein L17

Mitochondrial ribosomal protein L23

Mitochondrial ribosomal protein L38

Mitochondrial ribosomal protein L45

Mitochondrial ribosomal protein S18B

Mitochondrial ribosomal protein S18C

Mitochondrial ribosomal protein S23

Mki67 (FHA domain) interacting nuclear phosphoprotein

Profilin 2

Ribosomal protein S8

Ribosomal protein S29

SDA1 domain containing 1

Seryl-tRNA synthethase

Slow myosin heavy chain 2

Small nuclear ribonucleoprotein D1 polypeptide

Small nuclear ribonucleoprotein D3 polypeptide

Small nuclear ribonucleoprotein polypeptide E

Small nuclear ribonucleoprotein polypeptide F-like

Thrombospondin 1

Thrombospondin 4b

Titin a

Tubulin, alpha 8 like 2

Tubulin, alpha 8 like 4

Tubulin, beta 5

Vessicle-associated membrane protein, associated protein B and C

Annotation Cluster 2

ATP synthase, H+ transporting, mitochondrial F0 complex, subunit b

(Enrichment Score: 4.69)

ATP synthase, H+ transporting, mitochondrial F0 complex, subunit e

ATP synthase, H+ transporting, mitochondrial F0 complex, alpha subunit 1

ATPase, H+ transporting, lysosomal, V1 subunit H

NADH dehydrogenase (ubiquinone) 1 beta subcomplex 4

NADH dehydrogenase (ubiquinone) Fe-S protein 4

Chromatin modifying protein 5

COX17 cytochrome c oxidase assembly homolog

Cytochrome c oxidase subunit VIIa 2

Frataxin

Heat shock protein 9

Mitochondrial ribosomal protein 63

Mitochondrial ribosomal protein L15

Mitochondrial ribosomal protein L23

Mitochondrial ribosomal protein L45

Nuclear cap binding protein subunit 2

SDA1 domain containing 1

Solute carrier family 25, member 16

Succinate dehydrogenase complex, subunit A, flavoprotein

Succinate dehydrogenase complex, subunit B, iron sulfur

Succinate dehydrogenase complex, subunit D, integral membrane protein A

Succinate dehydrogenase complex, subunit D, integral membrane protein B

Succinate dehydrogenase complex, subunit A, flavoprotein

Transcription factor B2

Ubiquinol-cytochrome c reductase, Rieske iron-sulfur polypeptide 1