Figure 6

Median elemental abundances (quantified as moderated Z-scores) of clusters generated using exhaustive significance clustering (ESC) on the ionome of yeast mutants with loss of function of genes that have a significant effect on at least one element in the ionome are visualized using a heat map. The clusters including less than three genes are not shown. Clusters are represented in rows and elements are represented in columns. The dendrogram represents relationships between cluster (left) and elements (top) using hierarchical clustering. The grey-scale shading on the left of the heat map visually represents the number of genes in a cluster (darker represents more genes). Numbers on the heat map are moderated Z-scores for each element within each cluster. The highlight color of the numbers represents the magnitude of the abundance - green if the median elemental is less than −3.328, yellow if it is greater than 3.473, a gradient of blue if between −3.328 and 0, and a gradient of magenta if between 0 and 3.473. The yellow and green colors emphasize the elements that are positively (significance cut off = 3.473 moderated Z-scores) or negatively (significance cut of = −3.328 moderated Z-scores) changed in each of the clusters.