Figure 8

Visualization of the interaction network of selected genes for which loss of function impacts the ionome. (A) The 22 genes from CLUSTER_10 and CLUSTER_11 that increase the abundance of Mn (see Figure 6) were selected and an interaction network built based on known protein protein and genetic interactions. There is 1 physical interaction and 23 genetic interactions among 13 of the selected 22 genes. (B) The 51 genes that significantly affect the abundance (increase and decrease) of Mn were selected and an interaction network built based on known protein protein and genetic interactions. There are 4 physical interactions and 32 genetic interactions among 23 of the 51 selected genes. Protein protein and genetic interaction information were obtained from BioGRID (48). Nodes represent genes, node color represents the direction of changes in elemental abundance (magenta increase in abundance, blue decrease in abundance), and node size represents the magnitude of the change in the ionome based on moderated Z-score. Lines joining the nodes (edges) in the graph represent the interactions. The color of the edges represents the direction of the correction (pink is positive and green negative) between the interacting pair of genes based on the ionomic profiles of the loss of function mutants. The width of the edges represents the strength of the correlation. A dotted edge represents a genetic interaction and solid edge represents a physical interaction.