Figure 3

RNA-seq and qPCR confirm trends in transcriptional changes between strains that differ in their tick transmission status. A. Fold change in the transmissible St. Maries strain relative to the non-transmissible Florida strain for all promoter candidates expressed in log scale 10. Locus tags for all genes are given on the X axis. Blue bars show the results obtained after evaluating two biological replicates with RT-PCR. Red bars show the fold change obtained using RNA-seq analysis for the promoter candidates across two biological replicates. The asterisk indicates statistical significance at p < 0.05. B. Fold change in the transmissible St. Maries strain relative to the non-transmissible florida strain expressed in log₁₀. The top 18 differentially transcribed genes identified through RNA-seq across two replicates and two statistical tests and their fold changes are shown. Red bars show results obtained with RNA-seq, blue bars show validation through qPCR. The asterisk indicates statistical significance at p < 0.01.