Figure 4

Representative sample of sqRT-PCR evaluation of SCGB1A1 transcript levels in various equine tissues. SCGB1A1 (label “1”) and SCGB1A1A (label “A”) cDNAs were detected as 200 bp amplification bands. Equine GAPDH (label “G”) was amplified as an internal control (254 bp). Densely stained amplicons of SCGB1A1 and SCGB1A1A cDNA were detected in lung, uterus, Fallopian tube and mammary gland tissues. Faint bands were present in multiple tissues including brain, pituitary, eye, nose epithelium, tongue, salivary gland, trachea, aorta, cardiac muscle, liver, spleen, small and large intestine, adrenal gland, kidney, skin, bladder, urethra, prostate, epididymis, seminal vesicle, testis and ovary. No PCR products were detected with cDNA from the eyelid gland, thyroid, bone marrow, pancreas, stomach and lymph node. SI, small intestine; LI, large intestine; EG, eyelid gland; NE, Nose epithelium; SG, salivary gland; CM, cardiac muscle; LN, lymph node; BM, bone marrow; SV, seminal vesicle.