Figure 10From: 5′ tRNA halves are present as abundant complexes in serum, concentrated in blood cells, and modulated by aging and calorie restrictionReal-time PCR amplification plots of circulating miRNAs. Shown are the amplification plots for miR-16 (blue), miR-24 (black), and the spiked-in miR-Cel-39 (red) measured in mouse serum (A), mouse serum treated with EDTA (B), and mouse plasma collected on EDTA (C). The y-axis represents the relative fluorescence units (RFU) in a semi-log scale. The x-axis represents the cycle at which fluorescence was detected above an automatically determined threshold for the indicated miRNA. EDTA does not change the concentration of miRNAs in plasma.Back to article page