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Table 1 Read statistics of 454 cDNA sequences after quality filtering

From: BSTA: a targeted approach combines bulked segregant analysis with next- generation sequencing and de novo transcriptome assembly for SNP discovery in sunflower

Reads

Number of reads

Bases

BR

1,182,916

425,618,400

BS

1,177,524

426,564,503

Aligned*

2,200,571

785,888,510

(93.2%)

(92.2%)

Assembled*

1,981,006

54,877,004

Partially aligned*

219,291

-

Singletons*

133,795

-

Repeats*

471

-

Outliers*

24,280

-

  1. *Definitions of these Newbler de novo assembly terms can be found in Additional file 5: Text S1.
  2. Sequence libraries of one resistant bulk (BR) and one susceptible bulk (BS) each containing pooled cDNA of 16 individual F2 plants were generated to perform the 454 sequencing.
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BMC Genomics

ISSN: 1471-2164

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