Figure 3

Coverage across the genome. The mean coverage along the w Bm genome ( Panels A and B ) or a similarly sized Brugia contig ( Panels C and D ) are plotted using the 300 bp paired end reads ( Panels A and C ) and the 3 kbp mate pair reads ( Panels B and D ). The mean coverage was calculated using 10-bp windows and is presented on a log scale. The dashed red line indicates the cut-off used for detecting nuwts with BWA, which was 16× for the paired end reads and 3× for the mate pair reads. Wolbachia w Bm regions having a mean coverage above this cut-off are identified as nuwts located in the genome of B. malayi.