Table 4 qPCR-based copy number for 10 nuwt fragments, compared to the corresponding coverage-based copy number

Wbm0022

alpha/beta fold family hydrolase

6-8

773 ± 63

2.5 ± 0.7 (n = 6)

3-9

Wbm0078

4'-phosphopantetheinyl transferase

5

793 ±42

2.5 ± 0.2 (n = 3)

5-6

Wbm0147

thiol-disulfide isomerase

2-5

287 ± 59

2.4 ± 0.2 (n = 3)

5-6

Wbm0240

HIT family hydrolase

4-6

712 ± 119

2.2 ± 0.4 (n = 3)

3-6

Wbm0241

NADH:ubiquinone oxidoreductase chain A

4

530 ± 22

−0.1 ± 0.2 (n = 3)

1

Wbm0275

glutamine synthetase

2

293 ± 15

−1.7 ± 0.2 (n = 3)

<1

Wbm0429

DNA polymerase III beta clamp subunit

10-12

1288 ± 79

2.5 ± 0.5 (n = 6)

4-8

Wbm0502

50S ribosomal protein L9

3-6

646 ± 188

2.6 ± 0.3 (n = 6)

5-7

Wbm0784

hypothetical protein

3-4

416 ± 21

2.1 ± 0.2 (n = 3)

4-5

Wbm0797

type IV secretion system ATPase VirB4

1-15

162 ± 27

−0.2 ±0.3 (n = 3)

1