Custom microarray construction and analysis for determining potential biomarkers of subchronic androgen exposure in the Eastern Mosquitofish (Gambusia holbrooki)

Table 2 Significantly differentially regulated biological processes during TB treatment as determined by gene set enrichment analysis

Gene Ontology Biological Process	Fisher’s Raw p-value	Type of regulation	Dif. reg^a	Not dif. reg^b
go:0006629; lipid metabolic process	0.002	Enriched	1.36%	0.72%
go:0008380; rna splicing	0.047	Enriched	1.18%	0.76%
go:0015986; atp synthesis coupled proton transport	0.001	Enriched	0.73%	0.26%
go:0051246; regulation of protein metabolic process	0.018	Enriched	0.47%	0.17%
go:0051028; mrna transport	0.025	Enriched	0.37%	0.13%
go:0030168; platelet activation	0.046	Enriched	0.36%	0.15%
go:0032313; regulation of rab gtpase activity	0.046	Enriched	0.36%	0.15%
go:0042157; lipoprotein metabolic process	0.0001	Enriched	0.35%	0.02%
go:0006695; cholesterol biosynthetic process	0.0005	Enriched	0.30%	0.02%
go:0030301; cholesterol transport	0.013	Enriched	0.24%	0.04%
go:0008203; cholesterol metabolic process	0.004	Enriched	0.18%	0%
go:0033344; cholesterol efflux	0.022	Enriched	0.18%	0.02%
go:0045727; positive regulation of translation	0.040	Enriched	0.16%	0.02%
go:0006044; n-acetylglucosamine metabolic process	0.032	Enriched	0.12%	0%
go:0046677; response to antibiotics	0.032	Enriched	0.12%	0%

a. The percent of transcripts in the GO category which were differentially regulated.
b. The percent of transcripts in the GO category which were not differentially regulated.

ISSN: 1471-2164