Table 3 Real time Primers used for microarray validation
Putative gene annotation | Sequence identifier | Primer sequence 5’->3’ | Amplicon lenght (bp) | PCR efficiency |
|---|---|---|---|---|
N.D | rud_dec_c1521 | F-GCACTTGTTGGTGGTCCTTATTGCTGG | 183 | 92% |
R-TGATTTGGTTAGTCAACTTCGCCG | ||||
Sialic acid-binding lectin | rud_dec_c29141 | F-CGTGGGCAGAACCTTTCAGTATGAG | 98 | 94% |
R-CACTTCACCCACACCCGTTGTCCTT | ||||
Pyridine nucleotide-disulphide | rud_dec_c15726 | F-CCTGGGGAGCCGTCATCATTAGC | 149 | 91% |
R-TACCTCCCCTTCTCTTCCCAAAACAA | ||||
Tetraspanin, | rud_dec_c39872 | F-TGGGTTCGGTAGTTTTGTCCTTCTAGTC | 153 | 95% |
R-AAAACTGCTGCTGTAATACCACCCGAG | ||||
Epididymal secretory protein 1 | rud_dec_c2016 | F-ACCTGTTCCATTTCCCGTTCCCT | 149 | 95% |
R-GGACTTCCCACTTTACAAGCAGCCG | ||||
Methionine sulfoxide reductase | rud_dec2_c944 | F-AGATACCAAGTTCAACTCCCACTCGG | 261 | 89% |
R- CATCAGCCAGTGTTACGCTTTC | ||||
X- box binding protein 1 | rud_dec2_c33 | F-CAAGCAATCGCAAATCGCCAACA | 225 | 92% |
R-GTGGGAGACACTTTAAGTTGACCAG | ||||
Fructose-bisphosphatase | rud_dec_c1253 | F-AAGCAGCGGAACAGGCTAAAGAG | 199 | 90% |
R-CTGCCAGTGCTCTAAATGCCTTGTT | ||||
Inhibitor of apoptosis protein | rud_dec_c1093 | F-ATTGCCTGTGGTCACATGGTTA | 110 | 96% |
R-AGACAGCCATAAGAGCACGGACA | ||||
Glucose dehydrogenase | rud_dec_c2753 | F-TGGGAATGTTTCGTTCGTCACCT | 146 | 93% |
R-GAGGCATTCAACAACTCGAACC | ||||
ND | rud_dec_c27604 | F-GAATACTGCTTGTTGCTTTCGGTGT | 102 | 95% |
R-TGCCTCTCACTTCGTCTGTGTCGGA | ||||
Calmodulin | rud_dec_c873 | F-TGAAGTTGTATGCTGACGGAAATGGA | 111 | 95% |
R-TTGGAATACTTCAAGTAACCCCTCTTCACTA |