Figure 8

Regulation of the Sry1 promoter by Sryα and Sryγ. A) Sryα (blue) and Sryγ (green) transfected CHO cells significantly repress the Sry1 promoter construct when compared to empty vector control (black) or each other (bar above Sryα and Sryγ). B) Sryα and mutations of Sryα at amino acids 76 (Sryα P76T) and 38 (Sryα H38Q) transfected into CHO cells all repress the Sry1 promoter construct when compared to empty vector control (black), with the P76T and not H38Q significantly differing from Sryα. C) Sryγ and mutations of Sryγ at amino acids 76 (Sryγ T76P) and 38 (Sryγ Q38H) transfected into CHO cells all repress the Sry1 promoter construct when compared to empty vector control (black), with the T76P and Q38H significantly differing from Sryγ. For all figures error bars represent the standard error of the mean and statistical significance is shown as ∗ = p ≤ 0.05, ∗∗ = p ≤ 0.01, ∗∗∗ = p ≤ 0.001.