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Table 1 Theridion californicum and T. grallator transcriptome sequencing and assembly statistics

From: De novo characterization of the gene-rich transcriptomes of two color-polymorphic spiders, Theridion grallator and T. californicum (Araneae: Theridiidae), with special reference to pigment genes

Input

T. californicum

T. californicum

T. grallator

T. grallator

“Yellow”

“Colored”

“Yellow”

“Colored”

Initial reads 1 RNA-seq

165,289,830

166,918,608

219,423,001

297,051,726

Initial reads 1 ncDNA

111,126,430

102,037,432

241,426,000

187,430,684

Total initial reads 1

276,416,260

268,956,040

460,849,001

484,482,410

Preprocessed reads

141,712,102

109,218,670

88,114,377

151,149,116

Combined reads

250,930,772

239,263,493

Reads entering assembly 2

171,894,396

168,943,057

Inchworm K mers

885,888,079

820,735,751

Chrysalis contigs

5,658,477

6,140,420

Butterfly contigs

128,391 (389,967)3

104,481 (459,452)

Butterfly components (“Genes”)

83,701 (295,585)

89,166 (427,020)

Mean contig length (bp)

606 (289)

601 (235)

Median contig length (bp)

344 (152)

332 (130)

N50 contig length (bp)

901 (429)

926 (273)

Mean coverage depth (reads) 4

2284 (1722)

4493 (3001)

Median coverage depth (reads)

99 (33)

329 (45)

Maximum transcript length (bp)

24,235

17,866

  1. 1All counts are expressed as “single” reads for comparative purposes.
  2. 2Subset due to RAM limitations.
  3. 3Values outside parentheses are for contigs > 200 bp, inside parentheses contigs > 100 bp.
  4. 4Coverage calculated using RSEM [31].