Figure 3

qPCR expression values for candidate reference genes in grapevine samples. Two segregants from the Ruby x Sultanina crossing (112 and 19) in three phenological stages (anthesis, fruit-setting and 6–8 mm berries) treated or not with gibberellic acid (GA3) were used. These segregants represent extreme phenotypes for berry size. For relative expression the genes were normalized with the lowest expression gene. A, AIG1 (VvAIG1); B, T-complex protein 1 subunit beta (VvTCPB); C, vacuolar sorting-associated protein 4 (VvSAP4); D, 26S proteasome non-ATPase regulatory subunit 13 (VvPRN26S); E, carbon catabolite repressor protein 4 homolog 2 (VvCCRP); F, unkown protein function (VvUNP2); G, unkown protein function (VvUNP); H, unkown protein function (VvUNP3); I, Rab GDP dissociation inhibitor alpha (VvRABI); J, proactivator polypeptide-like 1 (VvPP1); K, acting-depolymerizing factor 2 (VvADF2); L, 26S protease regulatory subunit 4 homolog (VvPR26S). Other putative housekeeping genes reported and used in many works are the following: M, polyubiquitin (VvUBQ10, GenBank acc CB977307); N, plasma membrane intrinsic protein 2B (VvPIP2B, GenBank acc EC969993); and O, elongation factor 1-alpha (VvEF1-α, GenBank acc CB977561). Bars in the graphs correspond to standard error (SE) from three biological samples, assayed in duplicate. Different letters represent significant differences a t P < 0.05 by LSD test.