Figure 3
From: Biased gene expression in early honeybee larval development
Hexamerin 70b expression biases caste determination towards worker fate. A. Relative expression of the four hexamerin genes in 60 hour queen and worker larvae as determined by RT-qPCR. Error bars represent the standard error of the mean. Statistical significance was assessed using a one-way ANOVA, *** indicates P < 0.001. B. Relative expression of hex70b was determined 12 – 18 hours following injection of either hex70b or eGFP (control) dsRNA using RT-qPCR. Error bars represent the standard error of the mean. Statistical significance was assessed using a one tailed t-test. C. The phenotype of emerging bees was assessed using a suite of morphological characteristics (refer to Additional file 1 for more detail). Graph shows the percentage of individuals that developed as queen-like after RNAi against hex 70b or eGFP (control). Statistical significance was assessed using a t-test, * indicates P < 0.05.