Transcriptomic analysis of the lesser spotted catshark (Scyliorhinus canicula) pancreas, liver and brain reveals molecular level conservation of vertebrate pancreas function

Table 1 Assembly characteristics

Tissue(s)	Number of contigs >300 bp	Contig N50 (bp)	Longest contig (bp)	Mean ORF length (bp)	Max ORF length (bp)	Number ORFs with signal peptide
Pancreas only	6551	429	5386	177	3005	257 (3.92%)
Brain only	21241	798	16085	338	13751	1029 (4.84%)
Liver only	3423	676	13906	271	13532	220 (6.43%)
Pancreas, brain	3847	1479	10710	482	10394	210 (5.46%)
Pancreas, liver	924	1039	5907	329	4505	62 (6.71%)
Brain, liver	2482	1329	12080	481	10403	149 (6.00%)
Pancreas, liver, brain	6326	2257	11135	705	9329	356 (5.63%)

Tissue distribution of transcripts was assigned by read mapping, taking a value of ≥1 fragments per kilobase per million mapped reads (FPKM) as evidence of expression.

ISSN: 1471-2164